Matthew Leevy, Ph.D.




College of Arts and Sciences




University of Notre Dame Graduate School


Biomedical imaging is an important technique that can be used for several applications such as cancer research and cardiology. A range of imaging technology, such as PET, SPECT, micro-CT and optical X-ray, is available for imaging. However, many research institutes use rats and mice for preclinical experiments. The purpose of this study was to determine if Zebrafish are compatible for use in pre-clinical imaging, and which modalities and probes work best. Different Zebrafish specimens were tested using five different modalities and four probes. In order to assess with two-dimensional modalities, both fluorescence and planar X-Ray were performed on the specimens. The fluorescence imaging was acquired using OsteoSense 750x and ProSense 750x as the probes. Through the results, we discovered that OsteoSense did not work as well as the ProSense. The next three modalities represented three-dimensional imaging. These modalities consist of X-ray Computed Tomography (CT), Positron Emission Tomography (PET), and Single Photon Emission Computed Tomography (SPECT). The standard settings of high dosage and low voltage were used to assess with X-ray Computed Tomography. Sodium Fluoride (NaF) and Fludeoxyglucose (FDG) were the two probes tested with PET imaging, while MDP with technetium-99 was used for SPECT. The majority of the probes were detected in the specimens, but not at the correct target.

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