Using Deletion Mapping to Locate Mutagen-Sensitivity Gene mus305 in the Drosophila melanogaster Genome
Poster Number
033
College
College of Arts and Sciences
Department
Biology
Faculty Mentor
Kathryn Kohl, Ph.D.
Abstract
All organisms experience DNA damage, and a variety of DNA repair mechanisms exist to combat this damage. The goal of this research was to localize the DNA repair gene mus305 in the Drosophila melanogaster genome. To localize mus305, complementation analysis was conducted using three deficiencies while assaying for mutagen-sensitivity. Specifically, two broods were created by crossing virgin female flies containing a known allele of mus305 to male flies containing one of three deficiencies. Brood 1 offspring were treated with water, which acted as the control, while Brood 2 offspring were treated with MMS. Progeny were scored, and then percent relative survival was calculated as the ratio of mutant to control flies in Brood 2, normalized to the same ratio in Brood 1. These data were used to narrow the potential genomic location of mus305. A candidate gene was identified in this refined region, and current research is focused on sequencing this gene in mus305 mutants.
Previously Presented/Performed?
Annual Drosophila Research Conference, Dallas, Texas, March 2019
Grant Support?
Supported by an SC INBRE grant from the National Institute for General Medical Sciences (NIH-NIGMS)
Start Date
12-4-2019 2:15 PM
End Date
April 2019
Using Deletion Mapping to Locate Mutagen-Sensitivity Gene mus305 in the Drosophila melanogaster Genome
Richardson Ballroom – DiGiorgio Campus Center
All organisms experience DNA damage, and a variety of DNA repair mechanisms exist to combat this damage. The goal of this research was to localize the DNA repair gene mus305 in the Drosophila melanogaster genome. To localize mus305, complementation analysis was conducted using three deficiencies while assaying for mutagen-sensitivity. Specifically, two broods were created by crossing virgin female flies containing a known allele of mus305 to male flies containing one of three deficiencies. Brood 1 offspring were treated with water, which acted as the control, while Brood 2 offspring were treated with MMS. Progeny were scored, and then percent relative survival was calculated as the ratio of mutant to control flies in Brood 2, normalized to the same ratio in Brood 1. These data were used to narrow the potential genomic location of mus305. A candidate gene was identified in this refined region, and current research is focused on sequencing this gene in mus305 mutants.
