Standard Dilution Analysis Applied to DART-MS: An Analysis of Caffeine
Poster Number
039
College
College of Arts and Sciences
Department
Chemistry, Physics, Geology, & the Environment
Faculty Mentor
Clifton Calloway, Ph.D.
Abstract
Caffeine is the most-used stimulant in America, with 90% of Americans consuming some form on a daily basis. Caffeine has been shown to have some health benefits, such as improving weight loss, increasing attention and brain function, and reducing the risk of Alzheimer’s and Parkinson’s diseases. There are, however, several reasons why limiting caffeine consumption could be beneficial. One way to limit intake is to know how much caffeine is in the beverages consumed, information often not printed on the container or label. Direct Analysis in Real Time Mass Spectrometry (DART-MS) is an ionization technique, in which ions are formed at atmospheric pressure, and mass spectra are produced in real time. A wide array of samples can be analyzed directly in this manner, eliminating most, if not all, sample preparation. DART-MS allows for fast and simple identification of compounds, but is not ideal for quantitative analysis due to fluctuations in ion formation. Using Standard Dilution Analysis (SDA) is one potential method to correct for these fluctuations. SDA is a relatively new calibration technique which combines the principles of standard addition and internal standardization. In this method, a series of dilutions allows for the analyte concentration of a sample to be determined using the mathematic relationship between internal standard and analyte signals. This work aims to enhance the quantitative capabilities of DART-MS, by applying SDA/DART-MS to determination of caffeine in commercial beverage samples. Additionally, this work aims to illuminate the advantages of SDA/DART-MS over other more traditional methods.
Course Assignment
CHEM 551, 552 – Hanna
Start Date
12-4-2019 2:15 PM
End Date
April 2019
Standard Dilution Analysis Applied to DART-MS: An Analysis of Caffeine
Richardson Ballroom – DiGiorgio Campus Center
Caffeine is the most-used stimulant in America, with 90% of Americans consuming some form on a daily basis. Caffeine has been shown to have some health benefits, such as improving weight loss, increasing attention and brain function, and reducing the risk of Alzheimer’s and Parkinson’s diseases. There are, however, several reasons why limiting caffeine consumption could be beneficial. One way to limit intake is to know how much caffeine is in the beverages consumed, information often not printed on the container or label. Direct Analysis in Real Time Mass Spectrometry (DART-MS) is an ionization technique, in which ions are formed at atmospheric pressure, and mass spectra are produced in real time. A wide array of samples can be analyzed directly in this manner, eliminating most, if not all, sample preparation. DART-MS allows for fast and simple identification of compounds, but is not ideal for quantitative analysis due to fluctuations in ion formation. Using Standard Dilution Analysis (SDA) is one potential method to correct for these fluctuations. SDA is a relatively new calibration technique which combines the principles of standard addition and internal standardization. In this method, a series of dilutions allows for the analyte concentration of a sample to be determined using the mathematic relationship between internal standard and analyte signals. This work aims to enhance the quantitative capabilities of DART-MS, by applying SDA/DART-MS to determination of caffeine in commercial beverage samples. Additionally, this work aims to illuminate the advantages of SDA/DART-MS over other more traditional methods.
