Event Title

Mapping Mutagen-Sensitivity of mus111 in Drosophila melanogaster

Session Title

Cancer and Biomedical Research

Faculty Mentor

Kathryn Kohl, Ph.D.

College

College of Arts and Sciences

Department

Department of Biology

Location

WEST 219

Start Date

12-4-2019 12:45 PM

Description

Drosophila melanogaster is an ideal genetic model organism that has orthologs of roughly 75% of human disease-causing genes. In this experiment I am using MMS, a DNA-damaging agent, to map hypersensitivity using a mutagen-sensitivity assay. I am using this assay in an attempt to locate the mutagen-sensitivity gene mus111 in Drosophila melanogaster. To accomplish this, I will use three different deletion stocks to cross with mus111 mutant flies. If a deletion uncovers the mus111 gene, mutagen sensitivity will occur. I will set up 30 vials containing four virgined females carrying one of three deletions and five males carrying the mus111 allele. From these vials, I will create two broods, one of which will be treated with water and the other with MMS. I will then perform a biological replicate by repeating the experiment before collecting my results to determine if mutagen-sensitivity occurred in any of the three deletions. Results from these experiments will be presented.

Grant Support?

Supported by an SC INBRE grant from the National Institute for General Medical Sciences (NIH-NIGMS)

Course Assignment

BIOL 471 – Kohl

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Apr 12th, 12:45 PM

Mapping Mutagen-Sensitivity of mus111 in Drosophila melanogaster

WEST 219

Drosophila melanogaster is an ideal genetic model organism that has orthologs of roughly 75% of human disease-causing genes. In this experiment I am using MMS, a DNA-damaging agent, to map hypersensitivity using a mutagen-sensitivity assay. I am using this assay in an attempt to locate the mutagen-sensitivity gene mus111 in Drosophila melanogaster. To accomplish this, I will use three different deletion stocks to cross with mus111 mutant flies. If a deletion uncovers the mus111 gene, mutagen sensitivity will occur. I will set up 30 vials containing four virgined females carrying one of three deletions and five males carrying the mus111 allele. From these vials, I will create two broods, one of which will be treated with water and the other with MMS. I will then perform a biological replicate by repeating the experiment before collecting my results to determine if mutagen-sensitivity occurred in any of the three deletions. Results from these experiments will be presented.