Mapping Mutagen-Sensitivity of mus111 in Drosophila melanogaster
Session Title
Cancer and Biomedical Research
College
College of Arts and Sciences
Department
Biology
Faculty Mentor
Kathryn Kohl, Ph.D.
Abstract
Drosophila melanogaster is an ideal genetic model organism that has orthologs of roughly 75% of human disease-causing genes. In this experiment I am using MMS, a DNA-damaging agent, to map hypersensitivity using a mutagen-sensitivity assay. I am using this assay in an attempt to locate the mutagen-sensitivity gene mus111 in Drosophila melanogaster. To accomplish this, I will use three different deletion stocks to cross with mus111 mutant flies. If a deletion uncovers the mus111 gene, mutagen sensitivity will occur. I will set up 30 vials containing four virgined females carrying one of three deletions and five males carrying the mus111 allele. From these vials, I will create two broods, one of which will be treated with water and the other with MMS. I will then perform a biological replicate by repeating the experiment before collecting my results to determine if mutagen-sensitivity occurred in any of the three deletions. Results from these experiments will be presented.
Course Assignment
BIOL 471 – Kohl
Grant Support?
Supported by an SC INBRE grant from the National Institute for General Medical Sciences (NIH-NIGMS)
Start Date
12-4-2019 12:45 PM
Mapping Mutagen-Sensitivity of mus111 in Drosophila melanogaster
WEST 219
Drosophila melanogaster is an ideal genetic model organism that has orthologs of roughly 75% of human disease-causing genes. In this experiment I am using MMS, a DNA-damaging agent, to map hypersensitivity using a mutagen-sensitivity assay. I am using this assay in an attempt to locate the mutagen-sensitivity gene mus111 in Drosophila melanogaster. To accomplish this, I will use three different deletion stocks to cross with mus111 mutant flies. If a deletion uncovers the mus111 gene, mutagen sensitivity will occur. I will set up 30 vials containing four virgined females carrying one of three deletions and five males carrying the mus111 allele. From these vials, I will create two broods, one of which will be treated with water and the other with MMS. I will then perform a biological replicate by repeating the experiment before collecting my results to determine if mutagen-sensitivity occurred in any of the three deletions. Results from these experiments will be presented.
