Decellularization and Recellularization of Porcine Acellular Muscle Matrix Scaffolds
College
College of Arts and Sciences
Department
Biology
Faculty Mentor
Matthew Stern, Ph.D.
Abstract
Traumatic injuries often result in significant damage to skeletal muscle tissue. Current methods for repairing damaged skeletal muscle are inadequate and associated with donor site morbidity, as they require a patient’s healthy tissue to be harvested in attempts to repair or replace damaged tissue. A variety of biomaterials that facilitate muscle regeneration/repair are in development; however, few are able to provide the structural and biochemical cues present in the tissue’s native scaffolding, its extracellular matrix. Here, we describe the production and initial characterization of a biomaterial we refer to as Porcine Acellular Muscle Matrix (PAMM), which is produced through the decellularization of sheets of porcine skeletal muscle. We also demonstrate that PAMM scaffolds can be efficiently recellularized with murine C2C12 myoblasts. This work sets the stage for us to 1) use PAMM scaffolds to test the myogenic potential of different stem cell populations in a three-dimensional in vitro culture system and 2) test the ability of unseeded or cell-seeded PAMM scaffolds to support skeletal muscle regeneration in vivo.
Grant Support?
Supported by grants from the National Institutes of Health IDeA Networks for Biomedical Research Excellence (NIH-INBRE) and Winthrop University Research Council
Start Date
21-4-2017 12:45 PM
Decellularization and Recellularization of Porcine Acellular Muscle Matrix Scaffolds
DiGiorgio Campus Center, Room 220
Traumatic injuries often result in significant damage to skeletal muscle tissue. Current methods for repairing damaged skeletal muscle are inadequate and associated with donor site morbidity, as they require a patient’s healthy tissue to be harvested in attempts to repair or replace damaged tissue. A variety of biomaterials that facilitate muscle regeneration/repair are in development; however, few are able to provide the structural and biochemical cues present in the tissue’s native scaffolding, its extracellular matrix. Here, we describe the production and initial characterization of a biomaterial we refer to as Porcine Acellular Muscle Matrix (PAMM), which is produced through the decellularization of sheets of porcine skeletal muscle. We also demonstrate that PAMM scaffolds can be efficiently recellularized with murine C2C12 myoblasts. This work sets the stage for us to 1) use PAMM scaffolds to test the myogenic potential of different stem cell populations in a three-dimensional in vitro culture system and 2) test the ability of unseeded or cell-seeded PAMM scaffolds to support skeletal muscle regeneration in vivo.
