Porcine Acellular Muscle Matrix Scaffolds Support Recellularization by Myogenic Cells
College
College of Arts and Sciences
Department
Biology
Faculty Mentor
Matthew Stern, Ph.D.
Abstract
Skeletal muscle has a remarkable, yet limited capacity for regeneration. Severely damaged skeletal muscle is incapable of full regeneration, leaving patients with few suitable options for restoring lost muscle mass and/or function. Tissue engineering and regenerative medicine offer a potential solution for individuals with severely damaged muscle. Two components required for successful skeletal muscle tissue engineering/regenerative medicine are a source of myogenic cells and a biomaterial capable of stimulating myogenesis in vivo and in vitro. In our lab, we are producing a biomaterial from decellularized porcine muscle, which we refer to as Porcine Acellular Muscle Matrix (PAMM). Our goal is to recellularize PAMM scaffolds with C2C12 myoblasts. We hypothesize that PAMM scaffolds have the ability to promote recellularization and complete myogenic differentiation by C2C12 myoblasts and other sources of myogenic cells. Our results indicate that decellularization of porcine muscle can be achieved via two different protocols: 1) a detergent-based method and 2) a method based on actin depolymerization and hypertonic/hypotonic shock. Additionally, we establish that PAMM scaffolds can support the infiltration and growth of C2C12 myoblasts. We are currently exploring the potential of adipose-derived stem cells (ADSCs) to contribute to myogenic differentiation within PAMM scaffolds, following exposure to culture conditions that are believed to enhance their myogenic potential.
Previously Presented/Performed?
South Carolina INBRE symposium, Columbia, South Carolina, August 2016; South Carolina Academy of Science Annual Meeting, Coastal Carolina University, March 2017
Grant Support?
Supported by grants from the National Institutes of Health IDeA Networks for Biomedical Research Excellence (NIH-INBRE)
Start Date
21-4-2017 3:45 PM
Porcine Acellular Muscle Matrix Scaffolds Support Recellularization by Myogenic Cells
DiGiorgio Campus Center, Room 114
Skeletal muscle has a remarkable, yet limited capacity for regeneration. Severely damaged skeletal muscle is incapable of full regeneration, leaving patients with few suitable options for restoring lost muscle mass and/or function. Tissue engineering and regenerative medicine offer a potential solution for individuals with severely damaged muscle. Two components required for successful skeletal muscle tissue engineering/regenerative medicine are a source of myogenic cells and a biomaterial capable of stimulating myogenesis in vivo and in vitro. In our lab, we are producing a biomaterial from decellularized porcine muscle, which we refer to as Porcine Acellular Muscle Matrix (PAMM). Our goal is to recellularize PAMM scaffolds with C2C12 myoblasts. We hypothesize that PAMM scaffolds have the ability to promote recellularization and complete myogenic differentiation by C2C12 myoblasts and other sources of myogenic cells. Our results indicate that decellularization of porcine muscle can be achieved via two different protocols: 1) a detergent-based method and 2) a method based on actin depolymerization and hypertonic/hypotonic shock. Additionally, we establish that PAMM scaffolds can support the infiltration and growth of C2C12 myoblasts. We are currently exploring the potential of adipose-derived stem cells (ADSCs) to contribute to myogenic differentiation within PAMM scaffolds, following exposure to culture conditions that are believed to enhance their myogenic potential.
