Production of Chemically-Induced Pluripotent Stem Cells from Adipose-Derived Stem Cells is Limited by Toxicity of the Base Medium and Individual Chemicals
College
College of Arts and Sciences
Department
Biology
Faculty Mentor
Matthew Stern, Ph.D.
Abstract
Chemically induced pluripotent stem (CiPS) cells are created without genetic reprogramming from adult cells, through the introduction of small molecules. CiPS represent an attractive source in obtaining stem cells for therapeutic use because they match the differential potential of embryonic stem cells, using an effective procedure and without having to destroy the blastocyst. Following work by Hou at el. (2013), we hypothesized that CiPS cells would be created from ADSCs introduced to a seven-molecule cocktail. However, the ADSCs exposed to the seven molecules and ES medium experienced cell death. We then hypothesized that the base medium, solvents, and/or chemicals are not favorable to the growth conditions of the ADSCs. ADSCs were introduced separately to ethanol, DMSO, and all seven chemicals in either ES or ADSC medium. Our results demonstrate that the ES medium, along with CHIR, Rep Sox, and Forskolin individually, caused unhealthy morphology and cell death in the ADSCs. Future work will seek to reprogram Oct-4 GFP MEFs and ADSCs along with using MACS to sort ADSCs based on SSEA-1 expression prior to spheroid formation. This would enable ADSCs to serve as a source to create CiPS cells, which would ultimately be helpful for skeletal muscle tissue engineering and regenerative medicine applications.
Grant Support?
Supported by grants from the National Institutes of Health IDeA Networks for Biomedical Research Excellence (NIH-INBRE)
Start Date
21-4-2017 1:45 PM
Production of Chemically-Induced Pluripotent Stem Cells from Adipose-Derived Stem Cells is Limited by Toxicity of the Base Medium and Individual Chemicals
DiGiorgio Campus Center, Room 220
Chemically induced pluripotent stem (CiPS) cells are created without genetic reprogramming from adult cells, through the introduction of small molecules. CiPS represent an attractive source in obtaining stem cells for therapeutic use because they match the differential potential of embryonic stem cells, using an effective procedure and without having to destroy the blastocyst. Following work by Hou at el. (2013), we hypothesized that CiPS cells would be created from ADSCs introduced to a seven-molecule cocktail. However, the ADSCs exposed to the seven molecules and ES medium experienced cell death. We then hypothesized that the base medium, solvents, and/or chemicals are not favorable to the growth conditions of the ADSCs. ADSCs were introduced separately to ethanol, DMSO, and all seven chemicals in either ES or ADSC medium. Our results demonstrate that the ES medium, along with CHIR, Rep Sox, and Forskolin individually, caused unhealthy morphology and cell death in the ADSCs. Future work will seek to reprogram Oct-4 GFP MEFs and ADSCs along with using MACS to sort ADSCs based on SSEA-1 expression prior to spheroid formation. This would enable ADSCs to serve as a source to create CiPS cells, which would ultimately be helpful for skeletal muscle tissue engineering and regenerative medicine applications.
