Establishing a Novel 3D Tissue Culture System to Study Osteogenesis In Vitro
Poster Number
08
College
College of Arts and Sciences
Keywords
Osteoblasts, osteogenesis, three-dimensional, scaffolds, in vitro, seeding, cell, tissue
Department
Biology
Faculty Mentor
Dr. Mathew M. Stern
Abstract
The invention of 3D printers has made the process of engineering patent-specific prosthetic limbs less complex and more cost-efficient. While this represents a huge step forward for individuals that are in need of these limbs, several problems arise due to the non-biological nature of the prosthetics. In order for a recipient to obtain a limb that will mature and grow as they do, the fields of tissue engineering and regenerative medicine must make major advances in their ability to deliver clinical scale composite tissues. This requires an improved understanding of how different types of cells behave in a three-dimensional system. However, for decades, the in vitro study of cells, including bone cells, has been based on traditional two-dimensional cell culture. We hypothesized that 3D-printed scaffolds could provide a cost-efficient model to study osteogenesis in a three-dimensional in vitro system. To test this hypothesis, we printed three-dimensional scaffolds to the precise dimensions of sheep trabecular bone, functionalized the scaffolds with collagen I and hydroxyapatite coatings, and seeded them with a murine osteoblast cell line. Our results showed that 1) the scaffolds are biocompatible with the osteoblasts, 2) osteoblasts can adhere to and proliferate on the scaffolds, and 3) approximately 50% of seeded cells are incorporated into the scaffold during an initial round of seeding. Our results suggest that this method, with further optimization, can serve as a useful model to better understand the regenerative potential of bone cells and/or populations of stem cells with osteogenic potential in a three-dimensional system.
Previously Presented/Performed?
Summer Undergraduate Research Experience (SURE) Symposium, Winthrop University, July 2015
National Conference on Undergraduate Research (NCUR), Asheville, North Carolina, April 2016
Grant Support?
Supported by grants from the National Institutes of Health IDeA Networks for Biomedical Research Excellence (NIH INBRE) and the Winthrop University Research Council
Start Date
22-4-2016 12:00 PM
End Date
22-4-2016 2:00 PM
Establishing a Novel 3D Tissue Culture System to Study Osteogenesis In Vitro
Rutledge
The invention of 3D printers has made the process of engineering patent-specific prosthetic limbs less complex and more cost-efficient. While this represents a huge step forward for individuals that are in need of these limbs, several problems arise due to the non-biological nature of the prosthetics. In order for a recipient to obtain a limb that will mature and grow as they do, the fields of tissue engineering and regenerative medicine must make major advances in their ability to deliver clinical scale composite tissues. This requires an improved understanding of how different types of cells behave in a three-dimensional system. However, for decades, the in vitro study of cells, including bone cells, has been based on traditional two-dimensional cell culture. We hypothesized that 3D-printed scaffolds could provide a cost-efficient model to study osteogenesis in a three-dimensional in vitro system. To test this hypothesis, we printed three-dimensional scaffolds to the precise dimensions of sheep trabecular bone, functionalized the scaffolds with collagen I and hydroxyapatite coatings, and seeded them with a murine osteoblast cell line. Our results showed that 1) the scaffolds are biocompatible with the osteoblasts, 2) osteoblasts can adhere to and proliferate on the scaffolds, and 3) approximately 50% of seeded cells are incorporated into the scaffold during an initial round of seeding. Our results suggest that this method, with further optimization, can serve as a useful model to better understand the regenerative potential of bone cells and/or populations of stem cells with osteogenic potential in a three-dimensional system.
