Event Title

Standard Dilution Analysis Applied to DART-MS: An Analysis of Caffeine

Poster Number

039

Faculty Mentor

Clifton Calloway, Ph.D.

College

College of Arts and Sciences

Department

Department of Chemistry, Physics, and Geology

Location

Richardson Ballroom – DiGiorgio Campus Center

Start Date

12-4-2019 2:15 PM

End Date

April 2019

Description

Caffeine is the most-used stimulant in America, with 90% of Americans consuming some form on a daily basis. Caffeine has been shown to have some health benefits, such as improving weight loss, increasing attention and brain function, and reducing the risk of Alzheimer’s and Parkinson’s diseases. There are, however, several reasons why limiting caffeine consumption could be beneficial. One way to limit intake is to know how much caffeine is in the beverages consumed, information often not printed on the container or label. Direct Analysis in Real Time Mass Spectrometry (DART-MS) is an ionization technique, in which ions are formed at atmospheric pressure, and mass spectra are produced in real time. A wide array of samples can be analyzed directly in this manner, eliminating most, if not all, sample preparation. DART-MS allows for fast and simple identification of compounds, but is not ideal for quantitative analysis due to fluctuations in ion formation. Using Standard Dilution Analysis (SDA) is one potential method to correct for these fluctuations. SDA is a relatively new calibration technique which combines the principles of standard addition and internal standardization. In this method, a series of dilutions allows for the analyte concentration of a sample to be determined using the mathematic relationship between internal standard and analyte signals. This work aims to enhance the quantitative capabilities of DART-MS, by applying SDA/DART-MS to determination of caffeine in commercial beverage samples. Additionally, this work aims to illuminate the advantages of SDA/DART-MS over other more traditional methods.

Course Assignment

CHEM 551, 552 – Hanna

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Apr 12th, 2:15 PM Apr 12th, 4:15 PM

Standard Dilution Analysis Applied to DART-MS: An Analysis of Caffeine

Richardson Ballroom – DiGiorgio Campus Center

Caffeine is the most-used stimulant in America, with 90% of Americans consuming some form on a daily basis. Caffeine has been shown to have some health benefits, such as improving weight loss, increasing attention and brain function, and reducing the risk of Alzheimer’s and Parkinson’s diseases. There are, however, several reasons why limiting caffeine consumption could be beneficial. One way to limit intake is to know how much caffeine is in the beverages consumed, information often not printed on the container or label. Direct Analysis in Real Time Mass Spectrometry (DART-MS) is an ionization technique, in which ions are formed at atmospheric pressure, and mass spectra are produced in real time. A wide array of samples can be analyzed directly in this manner, eliminating most, if not all, sample preparation. DART-MS allows for fast and simple identification of compounds, but is not ideal for quantitative analysis due to fluctuations in ion formation. Using Standard Dilution Analysis (SDA) is one potential method to correct for these fluctuations. SDA is a relatively new calibration technique which combines the principles of standard addition and internal standardization. In this method, a series of dilutions allows for the analyte concentration of a sample to be determined using the mathematic relationship between internal standard and analyte signals. This work aims to enhance the quantitative capabilities of DART-MS, by applying SDA/DART-MS to determination of caffeine in commercial beverage samples. Additionally, this work aims to illuminate the advantages of SDA/DART-MS over other more traditional methods.