Event Title

Exploring Interactions between RitR and PhPP in the Presence of DNA and Their Role in Iron Uptake in S. pneumonia

Poster Number

17

Faculty Mentor

Nicholas Grossoehme, Ph.D.

College

College of Arts and Sciences

Department

Department of Chemistry, Physics, and Geology

Location

Rutledge

Start Date

21-4-2017 12:00 PM

Description

Streptococcus pneumonia is a pathogenic bacterium that is responsible for a large portion of the pneumonia infections in the United States. This organism has a striking ability to survive in a wide range of oxidative environments, suggesting a well-developed oxygen sensory and response mechanism; iron plays a key role in this process. RitR and PhPP have been shown to play essential roles in the regulation and control of iron uptake in the cell. While it is established that interactions between these two proteins occur, it is not understood how. Our research looks to obtain an understanding of how those interactions occur and how the intake of iron can be controlled when sufficient intracellular levels have been reached. The interaction between these two proteins has been explored using techniques such as gel filtration chromatography and enzyme kinetic analysis. Ongoing fluorescence experiments are aimed at investigating the role DNA has in the interaction between the two proteins. Results of this research will allow scientists to have deeper understanding of iron regulation in S. pneumonia and may enable development of methods to control its virulent characteristics.

Grant Support?

Supported by a grant from the National Institutes of Health IDeA Networks for Biomedical Research Excellence (NIH-INBRE)

Course Assignment

CHEM 551, 552 – Hanna

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Apr 21st, 12:00 PM

Exploring Interactions between RitR and PhPP in the Presence of DNA and Their Role in Iron Uptake in S. pneumonia

Rutledge

Streptococcus pneumonia is a pathogenic bacterium that is responsible for a large portion of the pneumonia infections in the United States. This organism has a striking ability to survive in a wide range of oxidative environments, suggesting a well-developed oxygen sensory and response mechanism; iron plays a key role in this process. RitR and PhPP have been shown to play essential roles in the regulation and control of iron uptake in the cell. While it is established that interactions between these two proteins occur, it is not understood how. Our research looks to obtain an understanding of how those interactions occur and how the intake of iron can be controlled when sufficient intracellular levels have been reached. The interaction between these two proteins has been explored using techniques such as gel filtration chromatography and enzyme kinetic analysis. Ongoing fluorescence experiments are aimed at investigating the role DNA has in the interaction between the two proteins. Results of this research will allow scientists to have deeper understanding of iron regulation in S. pneumonia and may enable development of methods to control its virulent characteristics.