Event Title

Isolation, Purification, and Characterization of Soil Bacteriophages Asriel and Haimas

Poster Number

20

Faculty Mentor

Victoria Frost, Ph.D., and Kristi Westover, Ph.D.

College

College of Arts and Sciences

Department

Department of Biology

Location

Rutledge

Start Date

21-4-2017 12:00 PM

Description

The goal of this study was to isolate, purify, and characterize bacteriophages found in soil near Rock Hill, South Carolina, near Winthrop University. In conjunction with the HHMI Science Education Alliance – Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) Program, this research expands our understanding of the genomic diversity of bacteriophages in this region. Two novel bacteriophages, Asriel and Haimas, were isolated from the soil and characterized. Samples were enriched using Mycobacterium smegmatis and purified until uniform plaques were isolated. High titer lysates were prepared from pure culture and DNA was isolated for analysis for restriction enzyme digests. DNA was sequenced at Pittsburg State University (PSU), followed by annotation using DNA Master Software. Asriel is 74,594 base pairs in length, has approximately 142 genes, and is a member of Cluster E. Haimas is 68,296 base pairs in length, has approximately 100 genes, and is a member of Cluster B1.

Previously Presented/Performed?

American Society for Microbiology, South Carolina Branch Meeting, Florence, South Carolina, March 2017

Grant Support?

Supported by grant from the Howard Hughes Medical Institute for the SEA-PHAGES Program

Course Assignment

BIOL 271 – Westover and BIOL 203R – Frost

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Apr 21st, 12:00 PM

Isolation, Purification, and Characterization of Soil Bacteriophages Asriel and Haimas

Rutledge

The goal of this study was to isolate, purify, and characterize bacteriophages found in soil near Rock Hill, South Carolina, near Winthrop University. In conjunction with the HHMI Science Education Alliance – Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) Program, this research expands our understanding of the genomic diversity of bacteriophages in this region. Two novel bacteriophages, Asriel and Haimas, were isolated from the soil and characterized. Samples were enriched using Mycobacterium smegmatis and purified until uniform plaques were isolated. High titer lysates were prepared from pure culture and DNA was isolated for analysis for restriction enzyme digests. DNA was sequenced at Pittsburg State University (PSU), followed by annotation using DNA Master Software. Asriel is 74,594 base pairs in length, has approximately 142 genes, and is a member of Cluster E. Haimas is 68,296 base pairs in length, has approximately 100 genes, and is a member of Cluster B1.