Event Title

Meiofaunal Flatworm Diet Analysis via Diagnostic PCR

Poster Number

16

Faculty Mentor

Julian Smith III, Ph.D.

College

College of Arts and Sciences

Department

Department of Biology

Location

Rutledge

Start Date

21-4-2017 12:00 PM

Description

Studies of meiofaunal food webs are hindered because a scarce number of prey have features that allow identification and persist long enough in the predator’s digestive tract. PCR-based approaches have proven to be successful methods for identifying and amplifying prey sequences from the digestive tracts of predators. In this paper, we will report the effective use of prey-taxon-specific primers in diagnostic PCR to identify prey, to the species level, in meiofaunal flatworms. The predator species in focus will be Proschizorhynchella, a kalyptorhynch flatworm. The prey species in focus will be Amphipoda, commonly known as amphipods, and Decapoda, commonly known as decapods. An alignment of crustacean 18S rDNA sequences will be generated, and primers specific to amphipods and decapods, that have never been designed before, will be created. Continuation of this method will permit establishment of the marine meiofaunal food web at the species level.

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Apr 21st, 12:00 PM

Meiofaunal Flatworm Diet Analysis via Diagnostic PCR

Rutledge

Studies of meiofaunal food webs are hindered because a scarce number of prey have features that allow identification and persist long enough in the predator’s digestive tract. PCR-based approaches have proven to be successful methods for identifying and amplifying prey sequences from the digestive tracts of predators. In this paper, we will report the effective use of prey-taxon-specific primers in diagnostic PCR to identify prey, to the species level, in meiofaunal flatworms. The predator species in focus will be Proschizorhynchella, a kalyptorhynch flatworm. The prey species in focus will be Amphipoda, commonly known as amphipods, and Decapoda, commonly known as decapods. An alignment of crustacean 18S rDNA sequences will be generated, and primers specific to amphipods and decapods, that have never been designed before, will be created. Continuation of this method will permit establishment of the marine meiofaunal food web at the species level.