Event Title

Enhancing the Developmental Potential of Murine Adipose-Derived Mesenchymal Stem Cells

Poster Number

45

Faculty Mentor

Matthew Stern, Ph.D.

College

College of Arts and Sciences

Department

Biology

Location

Richardson Ballroom

Start Date

24-4-2015 1:20 PM

End Date

24-4-2015 2:50 PM

Description

Adipose-derived stem cells (ADSCs) are multipotent somatic stem cells obtained from the microvasculature of adipose tissue. ADSCs cannot match the differentiation potential of pluripotent embryonic stem cells (ES cells). However, previous studies have suggested that the non-traditional method of culturing ADSCs as three-dimensional spheroids can induce the expression of factors associated with pluripotency, including the transcription factor Oct-4. We hypothesize that non-traditional, three-dimensional spheroid culturing of ADSCs can upregulate the expression of several genes associated with pluripotency, as well as increase the differentiation potential of ADSCs. Here, we show that murine ES cells cultured in our lab maintain expression of genes associated with the pluripotent state and known to be expressed in ES cells, thereby validating our ES cell culture conditions for future studies. We also show that ADSCs cultured under traditional two-dimensional conditions do not express markers of pluripotency. Interestingly, the expression of several genes know to be expressed in populations of somatic stem cells does vary with the level of confluence of ADSCs and is also affected by medium supplementation with murine leukemia inhibitory factor (mLIF), which is used to maintain pluripotency in cultured murine ES cells. Future work will examine the expression of the same subset of genes in ADSCs cultured as three-dimensional spheroids in the presence/absence of mLIF and murine embryonic fibroblast feeder cells.

Comments

Presented at the South Carolina Academy of Science Annual Meeting, April 2015

Supported by a grant from the Winthrop University Research Council and an NIH-INBRE grant from the National Center for Research Resources and the National Institute of General Medical Sciences

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Apr 24th, 1:20 PM Apr 24th, 2:50 PM

Enhancing the Developmental Potential of Murine Adipose-Derived Mesenchymal Stem Cells

Richardson Ballroom

Adipose-derived stem cells (ADSCs) are multipotent somatic stem cells obtained from the microvasculature of adipose tissue. ADSCs cannot match the differentiation potential of pluripotent embryonic stem cells (ES cells). However, previous studies have suggested that the non-traditional method of culturing ADSCs as three-dimensional spheroids can induce the expression of factors associated with pluripotency, including the transcription factor Oct-4. We hypothesize that non-traditional, three-dimensional spheroid culturing of ADSCs can upregulate the expression of several genes associated with pluripotency, as well as increase the differentiation potential of ADSCs. Here, we show that murine ES cells cultured in our lab maintain expression of genes associated with the pluripotent state and known to be expressed in ES cells, thereby validating our ES cell culture conditions for future studies. We also show that ADSCs cultured under traditional two-dimensional conditions do not express markers of pluripotency. Interestingly, the expression of several genes know to be expressed in populations of somatic stem cells does vary with the level of confluence of ADSCs and is also affected by medium supplementation with murine leukemia inhibitory factor (mLIF), which is used to maintain pluripotency in cultured murine ES cells. Future work will examine the expression of the same subset of genes in ADSCs cultured as three-dimensional spheroids in the presence/absence of mLIF and murine embryonic fibroblast feeder cells.