Enhancing the Developmental Potential of Murine Adipose-Derived Mesenchymal Stem Cells
Poster Number
45
College
College of Arts and Sciences
Department
Biology
Faculty Mentor
Matthew Stern, Ph.D.
Abstract
Adipose-derived stem cells (ADSCs) are multipotent somatic stem cells obtained from the microvasculature of adipose tissue. ADSCs cannot match the differentiation potential of pluripotent embryonic stem cells (ES cells). However, previous studies have suggested that the non-traditional method of culturing ADSCs as three-dimensional spheroids can induce the expression of factors associated with pluripotency, including the transcription factor Oct-4. We hypothesize that non-traditional, three-dimensional spheroid culturing of ADSCs can upregulate the expression of several genes associated with pluripotency, as well as increase the differentiation potential of ADSCs. Here, we show that murine ES cells cultured in our lab maintain expression of genes associated with the pluripotent state and known to be expressed in ES cells, thereby validating our ES cell culture conditions for future studies. We also show that ADSCs cultured under traditional two-dimensional conditions do not express markers of pluripotency. Interestingly, the expression of several genes know to be expressed in populations of somatic stem cells does vary with the level of confluence of ADSCs and is also affected by medium supplementation with murine leukemia inhibitory factor (mLIF), which is used to maintain pluripotency in cultured murine ES cells. Future work will examine the expression of the same subset of genes in ADSCs cultured as three-dimensional spheroids in the presence/absence of mLIF and murine embryonic fibroblast feeder cells.
Start Date
24-4-2015 1:20 PM
End Date
24-4-2015 2:50 PM
Enhancing the Developmental Potential of Murine Adipose-Derived Mesenchymal Stem Cells
Richardson Ballroom
Adipose-derived stem cells (ADSCs) are multipotent somatic stem cells obtained from the microvasculature of adipose tissue. ADSCs cannot match the differentiation potential of pluripotent embryonic stem cells (ES cells). However, previous studies have suggested that the non-traditional method of culturing ADSCs as three-dimensional spheroids can induce the expression of factors associated with pluripotency, including the transcription factor Oct-4. We hypothesize that non-traditional, three-dimensional spheroid culturing of ADSCs can upregulate the expression of several genes associated with pluripotency, as well as increase the differentiation potential of ADSCs. Here, we show that murine ES cells cultured in our lab maintain expression of genes associated with the pluripotent state and known to be expressed in ES cells, thereby validating our ES cell culture conditions for future studies. We also show that ADSCs cultured under traditional two-dimensional conditions do not express markers of pluripotency. Interestingly, the expression of several genes know to be expressed in populations of somatic stem cells does vary with the level of confluence of ADSCs and is also affected by medium supplementation with murine leukemia inhibitory factor (mLIF), which is used to maintain pluripotency in cultured murine ES cells. Future work will examine the expression of the same subset of genes in ADSCs cultured as three-dimensional spheroids in the presence/absence of mLIF and murine embryonic fibroblast feeder cells.
